I am trying to go from 32 bit Vista to 64 bit Windows 7.

The 32 bit Vista was installed by mistake. My system can handle a 64 bit OS. Now I have finally found the time to upgrade. But the 64 bit intallation DVD can't be read from my OS. Any Advice?

Do I need to boot from that DVD drive? Will that work?

You'll have to boot the 64 bit DVD and clean install.(Backup your files first)

There is no upgrade path from Vista x86 to Win 7 x64. The Clean install is your only option as suggested by scudzilla.

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