Do you mean never use it again while the application is running? Or never use it when the application is run in the future?
If it is the latter, you could add a bool flag to your projects Properties Settings.settings which is initialised to false. Upon entering form1_Load for the first time, the flag would be checked to ensure it is false, allowing the code to execute. The flag is then updated inhibiting it from the code being executed in the future.
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